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mp 7601 ne pertm nuclear  (ATCC)


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    Structured Review

    ATCC mp 7601 ne pertm nuclear
    Mp 7601 Ne Pertm Nuclear, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+7601+ne+pertm+nuclear/Hs+865%2ESk/pm36995937-256-243-268
    Average 94 stars, based on 42 article reviews
    mp 7601 ne pertm nuclear - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Virus:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19

    Microarray:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19

    Recombinant:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19

    Mutagenesis:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19

    Polymer:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19

    Extraction:

    Article Title: CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-PRMT2 Thermo Fisher Cat# 720141; RRID:AB_2608486 Rabbit monoclonal anti-PRMT3 Abcam Cat# ab191562 Rabbit monoclonal anti-Seh1L Abcam Cat# ab218531 Rabbit polyclonal anti-PRMT7 ABclonal Cat# A12159; RRID:AB_2759045 Rabbit polyclonal anti-SEC13 ABclonal Cat# A11613; RRID:AB_27588636 Rabbit polyclonal anti-CDK1 ABclonal Cat# A0220; RRID:AB_2757034 Rabbit polyclonal anti-CDK2 ABclonal Cat# A0294; RRID:AB_2757106 Rabbit polyclonal anti- Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Epigentek Cat# A-3708 Rabbit polyclonal anti- Histone H4R3 Dimethyl Symmetric (H4R3me2s) Epigentek Cat# A-3718 Mouse monoclonal anti-HA BioLegend Cat# 901503; RRID:AB_2565005 Rabbit polyclonal anti-PRMT9 Bethyl Laboratories Cat# A304-189A; RRID:AB_2620386 Anti-aDMA Dr. Mark Bedford lab N/A Bacterial and virus strains XL10 Gold Escherichia coli Agilent Cat# 200314 BL21(DE3) Escherichia coli Sigma Aldrich Cat# CMC0014 Biological samples Liver cancer tissue microarray Biomax LV1021a Chemicals, peptides, and recombinant proteins Amino acids free RPMI medium United States Biological Cat# R8999-04A Furamidine dihydrochloride (FD) Tocris Bioscience Cat# 5202 TC-E 5003 Tocris Bioscience Cat# 5099 Anti-Flag agarose beads Sigma Aldrich Cat#A2220 Anti-HA agarose beads Sigma Aldrich Cat# A2095 Anti-Myc Tag affinity gel BioLedgend Cat# 658502 Glutathione Sepharose beads Cytiva Cat# 17075605 VECTASHIELD Antifade Mounting Media Vector Laboratories Cat# H-1400-10 Histone H4 recombination protein New England BioLabs Cat# M2504S GSK3368715 MedChemExpress Cat# HY-128717A SHURMount media General Data Cat# 682188 Adenosyl-L-Methionine, S-[methyl-3H] PerkinElmer Cat# NET155H250UC ATP, [g-32P] PerkinElmer Cat# NEG502Z250UC DSP Thermo Fisher Cat# 22585 Critical commercial assays QuikChange XL site-directed mutagenesis kit Agilent Cat# 20518 CellTiter-Glo Assay Kit Promega Cat# G9242 ImmPRESS Excel Amplifiered Polymer Kit Vector Laboratories Cat# MP-7601 NE-PERTM Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Cat# 78833 EpiQuik Total Histone Extraction Kit Epigentek Cat# OP-0006-100 Experimental models: Cell lines Human: HEK293 ATCC Cat# CRL-1573 Human: HEK293T ATCC Cat# CRL-3216 Human: Huh7 ATCC Cat# PTA-4583 Human: HepG2 ATCC Cat# HB-8065 Human: HeLa ATCC Cat# CCL-2 (Continued on next page) 18 Cell Reports 42, 112316, April 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Nude mice The Jackson Laboratory Strain # 007850 Oligonucleotides PRMTs sgRNAs Dr. Wenjian Gan laboratory Yin et al., 2021 CDK5-sg1: TGTGTTCAAGGCCAAAAACC This paper N/A CDK5-sg2: GGCCTTGAACACAGTTCCGT This paper N/A CDK5-sg3: GAGGCTGGATGACGATGATG This paper N/A WDR59-sg1: CGTTTTCGCTGCTCCATCGC This paper N/A WDR59-sg2: CTTTCGGTGACCTTCGAAAG This paper N/A DEPDC5-sg1: TAATACTCTTTTCAGAGTGG This paper N/A DEPDC5-sg2: CTTCATCCAGTATCCAGTGT This paper N/A NPRL2-sg1: CACCTTCAAGTGGATGGTGT This paper N/A NPRL2-sg2: GTCCAACACCATCCACTTGA This paper N/A KPTN-sg1: GCGCAACGGACAAGGCCCCG This paper N/A KPTN-sg2: GCAGAGCAATGTGTACGGGC This paper N/A WDR24-sg: CACGAACTGTTCCTCCTCGA This paper N/A ssODN for PRMT1 Flag tag knock-in: GAGGAGAAAGGGGGGGTCTTGGCG GCCGGAGGAGGAGTAGGTGCGGGT GAAGATGGACTACAAAGACCATGAC GGTGATTATAAAGATCATGACATTGA TTACAAGGATGACGATGACAAGGCG GCCGCAGGCGCGGCAGCCGAGGC TGCGAACTGCATCATGGAAGTGAGC GCTTGGAGCGCCGCCGTGGGCGGGAG This paper N/A ssODN for WDR24-R329K knock-in: TGT CTGGCTCCAAGGACAGCTCGCTGTGC CAGCACCTGTTCAAGGACGCCAGTCA GCCCGTCGAGCGCGCCAACCCTGAG GGCCTC This paper N/A Recombinant DNA Flag-PRMT1 This paper N/A HA-PRMT1 This paper N/A Myc-WDR24 This paper N/A pLenti-HA-WDR24 This paper N/A pLenti-HA-LAMP1 This paper N/A Tet-on-Flag-PRMT1 This paper N/A pGEX-PRMT1 This paper N/A pLJM1-Flag-RagBQ99L Addgene Cat# 19315 pLJM1-Flag-Rap2a Addgene Cat# 19311 HA-METAP2 Addgene Cat# 100512 HA-DEPDC5 Addgene Cat# 46327 HA-NPRL2 Addgene Cat# 99709 HA-NPRL3 Addgene Cat# 46330 HA-WDR59 Addgene Cat# 46328 HA-WDR24 Addgene Cat# 46335 HA-MIOS Addgene Cat# 46329 HA-SEH1L Addgene Cat# 46331 HA-SEC13 Addgene Cat# 46332 Flag-CASTOR1 Addgene Cat# 84488 (Continued on next page) Cell Reports 42, 112316, April 25, 2023 19



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